human oral squamous cell carcinoma oscc cell lines scc25  (ATCC)

Journal: BMC Oral Health

Article Title: Design and biological evaluation of peptide disruptors targeting YAP1-TEAD interaction for oral cancer

doi: 10.1186/s12903-025-07007-w

Figure Lengend Snippet: Systematic overview of developments and application of disruptors targeting YAP1-TEAD interaction in OSCC. Bioinformatic analysis of YAP1-TEAD and transcriptional target genes identified the importance of disrupting YAP1-TEAD in OSCC. A series of peptide disruptors that directly block YAP1-TEAD interactions were designed and optimized using computational modelling and in vitro binding experiments. Peptide disruptors with highest affinity were evaluated in OSCC cells and confirmed that the primary effect of disrupting YAP1-TEAD interactions in OSCC lies in suppressing cell migration and invasion

Article Snippet: The human OSCC cell lines SCC25 and HN30 (full names and details could be found in the supplementary materials) were originally purchased from Procell (China).

Techniques: Blocking Assay, In Vitro, Binding Assay, Migration

Journal: BMC Oral Health

Article Title: Design and biological evaluation of peptide disruptors targeting YAP1-TEAD interaction for oral cancer

doi: 10.1186/s12903-025-07007-w

Figure Lengend Snippet: YAP1-TEAD and four downstream transcriptional targets are significantly upregulated in OSCC. a Gene expression profiling of YAP1, TEAD1, TEAD2, TEAD3 and TEAD4 in OSCC tissue and the corresponding normal tissue with paired t-test. b Gene expression profiling of YAP1-TEAD downstream transcripts with significant differences in OSCC tissue and the corresponding normal tissue with paired t-test. c Kaplan-Meier survival analysis of YAP1-TEAD downstream transcripts with significant differences in high expressed and low expressed group. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The human OSCC cell lines SCC25 and HN30 (full names and details could be found in the supplementary materials) were originally purchased from Procell (China).

Techniques: Gene Expression

Journal: BMC Oral Health

Article Title: Design and biological evaluation of peptide disruptors targeting YAP1-TEAD interaction for oral cancer

doi: 10.1186/s12903-025-07007-w

Figure Lengend Snippet: YTPD9 and YTPD11 inhibited the abilities of migration and invasion in OSCC. a In SCC25 cells, YTPD11 reduced relative migration distance to 0.49 ± 0.068 after 24 h, while YTPD9 to 0.60 ± 0.11. b In HN30 cells, YTPD11 lowered relative migration distance to 0.66 ± 0.06, and YTPD9 was0.73 ± 0.05. c The invasion ability of HN30 cells was significantly inhibited by both disruptors. YTPD11 reduced the relative invasion ability to 0.23 ± 0.17, and YTPD9 to 0.23 ± 0.09. Additionally, compared to the spindle-shaped morphology of the cells in the control group, the cells treated with YTPD9 and YTPD11 exhibited a round-shaped morphology. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The human OSCC cell lines SCC25 and HN30 (full names and details could be found in the supplementary materials) were originally purchased from Procell (China).

Techniques: Migration, Control

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: Schematic graph showing the expected trends in Cornulin expression as dysplastic growth progresses toward malignant forms of oral squamous cell carcinoma. This original figure was drawn by authors using Microsoft Office PowerPoint and BioRender software.

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Expressing, Software

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: Cell line descriptions, special characteristics, culture media, and sources.

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Mutagenesis

Journal: Cureus

Article Title: Characterization of Cornulin as a Molecular Biomarker for the Progression of Oral Squamous Cell Carcinoma

doi: 10.7759/cureus.32210

Figure Lengend Snippet: (A) Western blot showing expression of Cornulin and the GAPDH loading control in the four cell lines tested. (B) Comparison of Cornulin expression relative to GAPDH expression in normal oral keratinocytes (PGK), dysplastic oral keratinocytes (DOK), locally invasive oral squamous cell carcinoma cells (SCC25), and metastatic oral squamous cell carcinoma cells (Detroit 562). Error bars represent standard error of the mean (SEM), and values shown above brackets indicate significant p-values pertaining to the difference of expression between cell lines. GAPDH, glyceraldehyde 3-phosphate dehydrogenase

Article Snippet: The squamous cell carcinoma 25 (SCC25) human OSCC cell line, Detroit 562 metastatic pharyngeal squamous cell carcinoma (SCC), and primary gingival epithelial cells were acquired from American Type Culture Collection (ATCC) (Manassas, VA), and the dysplastic oral keratinocytes (DOKs) were acquired from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, United Kingdom) via Millipore Sigma (Darmstadt, Germany).

Techniques: Western Blot, Expressing, Control, Comparison